CYTIVA CAPTO ADHERE MANUAL

The most appropriate material for column testing is, of course, the sample that is to be run in the application, but this is not always practical or economical. Locate the pressure relief valve between the air trap and the column. If this happens, fit an air trap and a pressure relief valve between the pump and column. Note that pressure spikes may cause poor packing (cracking). To check that the bed is stable, run the column at 70% packing pressure for 20 hours and test it again. If an initially poor result improves during a later test, the reason can be that the column was not properly equilibrated. Ideally, run three test runs to see whether the values are stable. It is important that the column is properly equilibrated ( >2 column volumes) before evaluating the packing.

These values can be determined easily by applying a NaCl or acetone solution, to the column (see below). They do not meet the above criteria and cannot be used with ion exchange and affinity chromatography media.Įxperience has shown that the best method of expressing the efficiency of a column is in terms of the height equivalent to a theoretical plate, HETP, reduced plate number, h, and the peak asymmetry factor, As. It is also an advantage if the same method can be used to monitor column performance over its working life, so that it is easy to determine when the medium should be re-packed or replaced.Īvoid methods that use colored compounds such as Blue Dextran. Such a method should be simple, quantitative and should not introduce contaminating materials. It is thus important to have a method by which the column can be tested before it is put into operation. A poorly packed column gives rise to uneven flow, resulting in zone broadening and reduced resolution. The efficiency of a column depends on how well it is packed. Materials of construction meet the ASME Bioprocessing Equipment Standard for biopharmaceutical manufacture.ĪxiChrom columns are supplied with comprehensive documentation to facilitate set-up, installation, and operation. The distribution system design has been developed using analytical methods and modern computational fluid dynamic modeling tools to ensure reproducible and predictable results over the entire range of scales. Packing large-scale AxiChrom columns (Video below)Įasy access to all column parts for maintenance in-situ is facilitated by the swing-out, hoist-free, column tube design. Both are controlled by the AxiChrom Master, a separate unit with a touch-screen interface that guides the operator through critical steps for priming, packing, unpacking, and maintenance. Intuitive handling supports preparation and maintenance procedures.

Intelligent Packing provides preprogrammed, verified axial compression packing methods that promote accurate and reproducible results. Proven, sanitary design meets stringent cGMP production standards.Swing-out, hoist-free design for easy maintenance and safer operation.Preprogrammed verified packing methods that include Capto™ and MabSelect™ media.Large-scale AxiChrom™ columns are supported with Intelligent Packing and intuitive handling via the AxiChrom Master.The novel, swing-out-design facilitates maintenance and provides a safer working enivironment. Comprehensive documentation, including IQ/OQ documents are available for all column sizes. The AxiChrom column is sanitizable, and materials of construction are suitable for use in regulated environments.

Scalable and predictable performance over the entire AxiChrom platform is assured through the use of computational fluid dynamics in designing the liquid distribution system.

CYTIVA CAPTO ADHERE MANUAL

The pivot design allows the column tube to be tilted for convenient manual emptying as well as easy access to parts for maintenance. The preprogrammed, verified packing methods shorten start-up time, eliminate errors and pack the column with optimal bed compression. Packing is facilitated by the Intelligent Packing methodology in the UNICORN software.

Operates with ÄKTAexplorer™ and ÄKTApilot™ with UNICORN™ v 5.01 (and later).ĪxiChrom columns are designed to simplify column handling and maintenance.

Proven sanitary design with construction materials recognized for use in GMP environments.

Rotating pivot stand for easy emptying, maintenance and safer operation.

Reproducible, predictable and scalable results.

Preprogrammed, verified packing methods that include Capto™, MabSelect™ media.

AxiChrom™ 50, 70, 100, 140, and 200 columns are designed to simplify column handling and maintenance during process development.

Cytiva capto adhere